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When utilizing IEM, i5 sequences must be entered into these program from the forward orientation in all circumstances and for all instrument types.
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Established A Index Adapters Make reference to Index 2 (i5) Orientation To learn more regarding how to enter i5 bases over the sample sheet in forward or reverse complement orientation.
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When demultiplexing, the final sample sheet supplied to your FASTQ technology software program should contain the i5 index in the correct orientation for that platform. Nonetheless, most Illumina sample sheet era program handles this automatically.
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NextSeq a thousand/2000 and NovaSeq X/X Additionally i5 indexes will correctly be output during the forward orientation for afterwards downstream processing. Customized kits also needs to hold the i5 indexes entered in the forward orientation.
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When evaluating index sets for color balance, the sequence noticed because of the instrument have to be assessed, regardless of the orientation used for the sample sheet. With the i7 index, generally asses colour balance utilizing the "i7 bases for sample sheet" column more info orientation.
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